Western blot analysis of GNAT1 Antibody in Jurkat cell line lysates (35ug/lane)

GNAT1 Antibody IHC analysis in formalin fixed and paraffin embedded moouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GNAT1 Antibody in Jurkat cell line lysates (35ug/lane)

GNAT1 Antibody

CATALOG NUMBER: 64-112

Clonality:

Polyclonal

Tested Applications:

Flow, IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Homology:

Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Xenopus

Immunogen:

This GNAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-318 amino acids from the C-terminal region of human GNAT1.

Conjugate:

Unconjugated

Tested Applications:

Flow, IHC-P, WB

Application Note:

For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50

Predicted Molecular Weight:

40 kDa

Advanced Validation

Properties

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

GNAT1

Additional Names:

Guanine nucleotide-binding protein G(t) subunit alpha-1, Transducin alpha-1 chain, GNAT1, GNATR

Protein Accession Number:

P11488

Protein Gi Number:

121032

Ncbi Gene Id Number:

2779

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells.

Background Reference 1:

Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008)

Background Reference 2:

Szabo, V., et al. Hum. Mutat. 28(7):741-742(2007)

Background Reference 3:

Yi, H.M., et al. Ai Zheng 26(1):9-14(2007)

Background Reference 4:

Oldham, W.M., et al. Nat. Struct. Mol. Biol. 13(9):772-777(2006)

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-112

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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GNAT1 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Homology:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

Customize your order

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