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42-684 (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-684 (3.8ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-684 (0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GNB3 Antibody

CATALOG NUMBER: 42-684

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: SGHDNRVSCLGVT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 37.2kDa according to NP_002066.1. Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebellum shows strong axonal staining in the Molecular layer and less so in the Granular layer. Recommended concentration, 3-5ug/ml.
Specificity:
This antibody is expected to recognize GNB3 & GNB4 (GeneID: 2784, 59345; ProteinID: NP_002066.1; NP_067642.1).
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GNB3
Additional Names:
GNB3, guanine nucleotide binding protein (G protein), beta polypeptide 3, G protein, beta-3 subunit, GTP-binding regulatory protein beta-3 chain, guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3, guanine nucleotide-binding protein, beta-3 subunit, hypertension associated protein, transducin beta chain 3
Protein Accession Number:
NP_002066.1,NP_067642.1
Protein Gi Number:
4504053
Ncbi Gene Id Number:
2784;59345

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-684

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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