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45-683 (1.0ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-683 (1.0ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GNB3 Antibody

CATALOG NUMBER: 45-683

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: DCMSLAVSPDFN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 36kDa band observed in Human Brain (Frontal Cortext) lysates (calculated MW of 37.2kDa according to NP_002066.1). Recommended concentration: 1-3ug/ml.
Specificity:
No cross-reactivity expected with GNB1, 2 and 4
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GNB3
Additional Names:
GNB3, guanine nucleotide binding protein (G protein), beta polypeptide 3, G protein, beta-3 subunit, GTP-binding regulatory protein beta-3 chain, guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3, guanine nucleotide-binding protein, beta-3 subunit, hypertension associated protein, transducin beta chain 3
Protein Accession Number:
NP_002066.1
Protein Gi Number:
4504053
Ncbi Gene Id Number:
2784
Background Reference 1:
Danoviz ME, Pereira AC, Mill JG, Krieger JE. Hypertension, obesity and gnb3 gene variants. Clin Exp Pharmacol Physiol. 2006 Mar;33(3):248-52.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-683

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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