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Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the detected GNE protein (arrow).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the detected GNE protein (arrow).

GNE Antibody

CATALOG NUMBER: 61-879

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Hamster, Rat
Immunogen:
This GNE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 580-607 amino acids from the C-terminal region of human GNE.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
79 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GNE
Additional Names:
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-GlcNAc-2-epimerase/ManAc kinase, UDP-N-acetylglucosamine 2-epimerase (hydrolyzing), UDP-GlcNAc-2-epimerase, Uridine diphosphate-N-acetylglucosamine-2-epimerase, N-acetylmannosamine kinase, ManAc kinase, GNE, GLCNE
Protein Accession Number:
Q9Y223
Protein Gi Number:
45476991
Ncbi Gene Id Number:
10020
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GNE is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells.
Background Reference 1:
Reinke, S.O., et al. Glycoconj. J. 26(4):415-422(2009)
Background Reference 2:
Tong, Y., et al. PLoS ONE(10), E7165 (2009)
Background Reference 3:
Klootwijk, R.D., et al. FASEB J. 22(11):3846-3852(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-879

List Size:
400 ul

List Price:

$495.00

Datasheet
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