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43-624 (0.03ug/ml) staining of K562 nuclear lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-624 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-624 (0.03ug/ml) staining of K562 nuclear lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

GNL3 Antibody

CATALOG NUMBER: 43-624

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-TENKAKSGKQNS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 65kDa band observed in nuclear lysates of cell line K562 (calculated MW of 62.0kDa according to NP_055181.3). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_055181.3; NP_996561.1). Reported variants represent identical protein: NP_996561.1, NP_996562.1.
Predicted Molecular Weight:
Approx 65kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GNL3
Additional Names:
GNL3, guanine nucleotide binding protein-like 3 (nucleolar), C77032, E2IG3, NNP47, NS, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide-binding protein-like 3, novel nucleolar protein 47, nucleolar GTP-binding protein 3, nucleostemin
Protein Accession Number:
NP_055181.3,NP_996561.1
Protein Gi Number:
7657048
Ncbi Gene Id Number:
26354
Background Reference 1:
Yoshida R, Fujimoto T, Kudoh S, Nagata M, Nakayama H, Shinohara M, Ito T.Nucleostemin affects the proliferation but not differentiation of oral squamous cell carcinoma cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-624

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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