Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of GNPDA1 in 293 cells with GNPDA1 antibody at 5 μg/mL.
Immunofluorescence of GNPDA1 in 293 cells with GNPDA1 antibody at 20 μg/mL.
Western blot analysis of GNPDA1 in mouse kidney lysate with GNPDA1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

GNPDA1 Antibody

CATALOG NUMBER: 5049

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
GNPDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GNPDA1.
The immunogen is located within the last 50 amino acids of GNPDA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
GNPDA1 is predicted to not cross-react with GNPDA2.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
GNPDA1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GNPDA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GNPDA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GNPDA1
Additional Names:
GNPDA1 Antibody: GPI, HLN, GNP1, GNPI, GNPDA, KIAA0060, Glucosamine-6-phosphate isomerase 1, Glucosamine-6-phosphate deaminase 1, GNPDA 1
Protein Accession Number:
AAC05123
Protein Gi Number:
2935438
Ncbi Gene Id Number:
10007
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GNPDA1 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA1 is one of two mammalian glucosamine-6-phosphate deaminases that are thought to have arisen though gene duplication, with the GNPDA2 protein closer in structure and activity to the E. coli enzyme. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.
Background Reference 1:
Wolosker H, Kline D, Bian Y, et al. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB J.1998; 12:91-9.
Background Reference 2:
Zhang J, Zhang W, Zou D, et al. Cloning and functional characterization of GNPI, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin. J. Cell Biochem.2003; 88:932-40.
Background Reference 3:
Arreola R, Valderrama B, Morante ML, et al. Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study. FEBS Lett.2003; 551:63-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5049

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369