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Western blot analysis of extracts of 293T cells, using GNRH2 antibody (23-574) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using GNRH2 antibody (23-574) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using GNRH2 antibody (23-574) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using GNRH2 antibody (23-574) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GNRH2 antibody (23-574) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of 293T cells, using GNRH2 antibody (23-574) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

GNRH2 Antibody

CATALOG NUMBER: 23-574

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-120 of human GNRH2 (NP_001492.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
293T
Predicted Molecular Weight:
Observed: 20kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GNRH2
Additional Names:
Progonadoliberin-2, Progonadoliberin II, Gonadoliberin-2, Gonadoliberin II, Gonadotropin-releasing hormone II, GnRH II, Luliberin II, Luteinizing hormone-releasing hormone II, LH-RH II, GnRH-associated peptide 2, GnRH-associated peptide II, GNRH2
Ncbi Gene Id Number:
2797
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a secreted peptide hormone and member of the gonadotropin-releasing hormone (GnRH) family of proteins. The encoded protein regulates reproductive function by stimulating the production and release of the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The encoded protein may inhibit endometrial, ovarian, prostate, and breast cancer cell proliferation. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-574

List Size:
100 uL

List Price:

$436.00

Datasheet
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