Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of GOLGA5 in human testis tissue lysate with GOLGA5 antibody at 1 μg/mL.
Immunohistochemistry of GOLGA5 in human testis tissue with GOLGA5 antibody at 5 μg/mL.
Immunofluorescence of GOLGA5 in human testis tissue with GOLGA5 antibody at 20 μg/mL.
Western blot analysis of GOLGA5 in human testis tissue lysate with GOLGA5 antibody at 1 μg/mL.
Trial Size

GOLGA5 Antibody

CATALOG NUMBER: 7387

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GOLGA5 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human GOLGA5.
The immunogen is located within amino acids 540 - 590 of GOLGA5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GOLGA5 antibody can be used for detection of GOLGA5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
GOLGA5 antibody is human, mouse and rat reactive. Two isoforms of GOLGA5 exist due to alternative splicing events.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Predicted Molecular Weight:
Predicted: 64, 80 kDa
Observed: 65, 78 kDa
Purification:
GOLGA5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GOLGA5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GOLGA5 antibody can be stored at 4°C, stable for up to one year.
Ncbi Official Symbol:
GOLGA5
Additional Names:
GOLGA5 Antibody: RFG5, GOLIM5, ret-II, RETII, RFG5, PIG31, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein
Protein Accession Number:
NP_005104
Protein Gi Number:
30260188
Ncbi Gene Id Number:
9950
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GOLGA5 Antibody: GOLGA5 (Golgi autoantigen, subfamily A, member 5), also known as GOLGA84, RFG5 (RET-fused gene 5 protein) or GOLIM5, is a ubiquitously expressed single-pass type II coiled-coil membrane protein that is involved in maintaining Golgi structure (1). It is localized to the Golgi apparatus and predominantly found on membranes at the cis side of the Golgi stack (1,2). As a mitotic phosphoprotein, GOLGA5 binds to active Rab 1 and associates with CASP (an isoform of CDP) in a golgin-tethering complex that is believed to play a role in intra-Golgi retrograde transport (3,4). Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) (5).
Background Reference 1:
Bascom RA, Srinivasan S, Nussbaum RL. Identification and characterization of golgin 84, a novel Golgi integral membrane protein with a cytoplasmic coiled-coil domain. J. Biol. Chem. 1999; 274:2953-62.
Background Reference 2:
Diao A, Rahman D, Pappin DJ, et al. 2003. The coiled-coil membrane protein golgin 84 is a novel Rab effector required for Golgi ribbon formation. J. Cell Biol. 2003; 160:201-12.
Background Reference 3:
Malsam J, Satoh A, Pelletier L, et al. Golgin tethers define subpopulations of COPI vesicles. Science 2005; 307:1095-8.
Background Reference 4:
Sohda M, Misumi Y, Yamamoto A, et al. Interaction of Golgin-84 with the COG complex mediates the intra-Golgi retrograde transport. Traffic 2010;11:1552-66.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7387

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369