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Western blot analysis of GOLGIN97 in human stomach tissue lysate with GOLGIN97 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking petpide.
Immunohistochemistry of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 5 μg/mL.
Immunofluorescence of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 20 μg/mL.
Western blot analysis of GOLGIN97 in human stomach tissue lysate with GOLGIN97 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking petpide.
Trial Size

GOLGIN97 Antibody

CATALOG NUMBER: 8215

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GOLGIN97 antibody was raised against a 17 amino acid peptide near the amino terminus of human GOLGIN97.
The immunogen is located within amino acids 30 - 80 of GOLGIN97.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GOLGIN97 antibody can be used for detection of GOLGIN97 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
GOLGIN97 antibody is human, mouse and rat reactive. At least two isoforms of GOLGIN97 are known to exist; this antibody will detect only the longer isoform. GOLGIN97 antibody is predicted to not cross-react with other GOLGIN family proteins.
Positive Control 1:
Cat. No. 1319 - Human Stomach Tissue Lysate
Predicted Molecular Weight:
Predicted: 84 kDa
Observed: 120 kDa
Purification:
GOLGIN97 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GOLGIN97 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GOLGIN97 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
GOLGA1
Additional Names:
Golgin subfamily A member 1, GOLGA1, GOLGIN A1, GOLGIN-97
Protein Accession Number:
NP_002068
Protein Gi Number:
4504063
Ncbi Gene Id Number:
2800
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GOLGIN97 was initially isolated as a Golgi complex autoantigen associated with Sjogren's syndrome, a chronic autoimmune disease in which the body's white blood cells destroy the exocrine glands, specifically the salivary and lacrimal glands (1,2). GOLGIN97 plays a role as a tethering molecule in endosome to the trans-Golgi network (TGN) retrograde traffic (3). FIP1/RCP binding to GOLGIN97 is required for tethering and fusion of recycling endosome retrograde transport vesicles to the TGN (4).
Background Reference 1:
Griffith KJ, Chan EK, Lung CC, et al. Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjogren's syndrome. Arthritis Rheum. 1997; 40:1693-702.
Background Reference 2:
Borchers AT, Naguwa SM, Keen CL, et al. Immunopathogenesis of Sjogren's syndrome. Clin. Rev. Allergy Immunol. 2003; 25:89-104.
Background Reference 3:
Lu L, Tai G, and Hong W. Autoantigen Golgin-97, and effector of ARL1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Mol. Biol. Cell 2004; 15:4426-43.
Background Reference 4:
Jing J, Junutula JR, Wu C, et al. FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-Golgi network. Mol. Biol. Cell 2010; 21:3041-53.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8215

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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