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Western blot analysis of GOLPH2 in rat brain tissue lysate with GOLPH2 antibody at (A) 0.25 and (B) 0.5 μg/mL.
Immunohistochemistry of GOLPH2 in rat brain tissue with GOLPH2 antibody at 2.5 μg/mL.
Immunofluorescence of GOLPH2 in rat brain tissue with GOLPH2 antibody at 20 μg/mL.
Western blot analysis of GOLPH2 in rat brain tissue lysate with GOLPH2 antibody at (A) 0.25 and (B) 0.5 μg/mL.
Trial Size

GOLPH2 Antibody

CATALOG NUMBER: 5441

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GOLPH2 antibody was raised against an 18 amino acid synthetic peptide from near the center of human GOLPH2.
The immunogen is located within amino acids 130 - 180 of GOLPH2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GOLPH2 antibody can be used for detection of GOLPH2 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 67 kDa
Observed: 70 kDa
Purification:
GOLPH2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GOLPH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GOLPH2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GOLM1
Additional Names:
GOLPH2 Antibody: GP73, HEL46, GOLPH2, C9orf155, PSEC0257, bA379P1.3, PSEC0242, UNQ686/PRO1326, Golgi membrane protein 1, Golgi membrane protein GP73
Protein Accession Number:
EAW62705
Protein Gi Number:
119583109
Ncbi Gene Id Number:
51280
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GOLPH2 Antibody: GOLPH2, also known as GOLM1, is a Golgi phosphoprotein that has a short cytoplasmic N-terminal domain, a membrane spanning region, and a longer C-terminal domain. It was initially identified as a possible marker for Alzheimer's disease, although later studies have demonstrated that the GOLPH2 gene does not contribute to risk of this disease. GOLPH2 expression has been reported higher in prostate cancer tissues compared to normal prostate tissue, suggesting that GOLPH2 can be used as an additional positive marker for tissue-based diagnosis of prostate cancer. It has been suggested that GOLPH2 expression in hepatocellular carcinomas (HCCs) and serum may also serve as tumor markers for HCCs.
Background Reference 1:
Kladney RD, Bulla GA, Guo L, et al. GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 2000; 249:53-65.
Background Reference 2:
Li H, Wetten S, Li L, et al. Candidate single-nucleotide polymorphisms from a genomewide association study of Alzheimer disease. Arch. Neurol. 2008; 65:45-53.
Background Reference 3:
Antunez C, Boada M, Lopez-Arrieta, et al. GOLPH2 gene markers are not associated with Alzheimer's disease in a sample of the Spanish population. J. Alzheimers Dis. 2009; 18:751-4.
Background Reference 4:
Kristiansen G, Fritzsche FR, Wassermann K, et al. GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: implications for tissue-based diagnostics. Br. J. Cancer 2008; 99:939-48.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5441

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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