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Western blot analysis of extracts of various cell lines, using GOT1 antibody (19-881) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat heart using GOT1 antibody (19-881) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using GOT1 antibody (19-881) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using GOT1 antibody (19-881) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using GOT1 antibody (19-881). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using GOT1 antibody (19-881) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

GOT1 Antibody

CATALOG NUMBER: 19-881

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
Jurkat
Positive Control 2:
293T
Positive Control 3:
HepG2
Positive Control 4:
A-549
Positive Control 5:
HeLa
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 46kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GOT1
Additional Names:
GOT1, GIG18, cCAT, cAspAT, ASTQTL1
Ncbi Gene Id Number:
2805
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-881

List Size:
100 uL

List Price:

$436.00

Datasheet
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