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Western blot analysis of extracts of HeLa cells, using GOT2 antibody (19-200).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of HeLa cells, using GOT2 antibody (19-200).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

GOT2 Antibody

CATALOG NUMBER: 19-200

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide of human GOT2
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
WB: 1:500 - 1:1000
Flow: 1:20 - 1:50
Positive Control 1:
HeLa
Predicted Molecular Weight:
Observed: 47kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GOT2
Additional Names:
GOT2, FLJ40994, kat4, kativ, mitaat, KAT4, KATIV, mitAAT
Ncbi Gene Id Number:
2806
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-200

List Size:
100 uL

List Price:

$365.00

Out of stock

Datasheet
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