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43-528 (0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-528 (0.1ug/ml) staining of Mouse Brain (A) and Heart (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-528 (0.3ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GOT2 Antibody

CATALOG NUMBER: 43-528

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: QGYRYYDPKTCGFD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 45kDa band observed in lysates of cell line HepG2, and of Mouse Brain and Heart (calculated MW of 47.5kDa according to human NP_002071.2 and 47.4kDa accordnig to mouse NP_034455.1). Recommended concentration: 0.3-1ug/ml.
Predicted Molecular Weight:
Approx 45kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GOT2
Additional Names:
GOT2, glutamic-oxaloacetic transaminase 2, mitochondrial aspartate aminotransferase 2 , FLJ40994, aspartate aminotransferase 2
Protein Accession Number:
NP_002071.2
Protein Gi Number:
73486658
Ncbi Gene Id Number:
2806
Background Reference 1:
Pol S, Bousquet-Lemercier B, Pave-Preux M, Pawlak A, Nalpas B, Berthelot P, Hanoune J, Barouki R.Nucleotide sequence and tissue distribution of the human mitochondrial aspartate aminotransferase mRNA.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-528

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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