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Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 μg/mL.
Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20 μg/mL.
Immunohistochemistry of GPAT1 in mouse brain tissue with GPAT1 antibody at 5 μg/ml.
Immunofluorescence of GPAT1 in mouse brain tissue with GPAT1 antibody at 20 μg/mL. <br><br>Green: GPAT1 Antibody (4613) <br> Blue: DAPI staining
Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

GPAT1 Antibody

CATALOG NUMBER: 4613

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (93%)
Immunogen:
GPAT1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1.
The immunogen is located within amino acids 730 - 780 of GPAT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of GPAT1 are known to exist; this antibody will detect only the two largest isoforms.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 87, 91 kDa
Observed: 92 kDa
Purification:
GPAT1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GPAT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GPAT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GPAM
Additional Names:
GPAT1 Antibody: GPAT, GPAT1, KIAA1560, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT-1
Protein Accession Number:
NP_065969
Protein Gi Number:
190358539
Ncbi Gene Id Number:
57678
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GPAT1 Antibody: Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets.
Background Reference 1:
Coleman RA and Lee DP. Enzymes of triacylglycerol synthesis and their regulation. Prog. Lipid Res. 2004; 43:134-76.
Background Reference 2:
Linden D, William-Olsson L, Ahnmark A, et al. Liver-directed overexpression of mitochondrial glycerol-3-phosphate acyltransferase results in hepatic steatosis, increased triacylglycerol secretion and reduced fatty acid oxidation. FASEB J. 2006; 20:434-43.
Background Reference 3:
Eberle D, Hegarty B, Bossard P, et al. SREBP transcription factors: master regulators of lipid homeostasis. Biochimie 2004; 86:839-48.
Background Reference 4:
Lewin TM, de Jong H, Schwerbrock NJ, et al. Mice deficient in glycerol-3-phosphate acyltransferase-1 have diminished myocardial triacylglycerol accumulation during lipogenic diet and altered phospholipid fatty acid composition. Biochim. Biophys. Acta 2008; 1781:352-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4613

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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