Western blot analysis of GPC6 using rabbit polyclonal GPC6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GPC6 gene.

GPC6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GPC6 using rabbit polyclonal GPC6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GPC6 gene.

GPC6 Antibody

CATALOG NUMBER: 55-977

Clonality:

Polyclonal

Tested Applications:

Flow, IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Immunogen:

This GPC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-528 amino acids from the C-terminal region of human GPC6.

Conjugate:

Unconjugated

Tested Applications:

Flow, IHC-P, WB

Application Note:

For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50

Predicted Molecular Weight:

63 kDa

Advanced Validation

Properties

Purification:

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

GPC6

Additional Names:

Glypican-6, Secreted glypican-6, GPC6

Protein Accession Number:

Q9Y625

Protein Gi Number:

9973298

Ncbi Gene Id Number:

10082

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

The glypicans comprise a family ofglycosylphosphatidylinositol-anchored heparan sulfateproteoglycans, and they have been implicated in the control of cellgrowth and cell division. The glypican encoded by this gene is aputative cell surface coreceptor for growth factors, extracellularmatrix proteins, proteases and anti-proteases. [provided byRefSeq].

Background Reference 1:

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Background Reference 2:

Lau, C.S., et al. BrOphthalmol 94(3):357-362(2010)

Background Reference 3:

Calboli, F.C., et al. PLoS ONE(7), E11504 (2010) :

Background Reference 4:

Wheeler, H.E., et al. PLoS Genet.(10), E1000685 (2009) :

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-977

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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GPC6 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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