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Western blot analysis of extracts of various cell lines, using GPD2 antibody (22-349) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver damage using GPD2 Antibody (22-349) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using GPD2 Antibody (22-349) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using GPD2 Antibody (22-349) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using GPD2 antibody (22-349) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

GPD2 Antibody

CATALOG NUMBER: 22-349

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-325 of human GPD2 (NP_000399.3).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
THP-1
Positive Control 3:
SW480
Positive Control 4:
Mouse skeletal muscle
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse pancreas
Predicted Molecular Weight:
Observed: 81kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GPD2
Additional Names:
Glycerol-3-phosphate dehydrogenase, mitochondrial, GPD-M, GPDH-M, mtGPD, GPD2
Ncbi Gene Id Number:
2820
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-349

List Size:
50 uL

List Price:

$295.00

Datasheet
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