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Antibody is used in Western blot to detect GPI8 in mouse liver tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect GPI8 in mouse liver tissue lysate.

GPI8 Antibody

CATALOG NUMBER: 61-397

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This GPI8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-297 amino acids from the C-terminal region of human GPI8.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PIGK
Additional Names:
GPI-anchor transamidase, GPI transamidase, 3---, GPI8 homolog, hGPI8, Phosphatidylinositol-glycan biosynthesis class K protein, PIG-K, PIGK, GPI8
Protein Accession Number:
Q92643
Protein Gi Number:
22001630
Ncbi Gene Id Number:
10026
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GPI8 is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
Background Reference 1:
Ohishi, K., et al., J. Biol. Chem. 278(16):13959-13967 (2003).
Background Reference 2:
Vainauskas, S., et al., J. Biol. Chem. 277(34):30535-30542 (2002).
Background Reference 3:
Ohishi, K., et al., EMBO J. 20(15):4088-4098 (2001).
Background Reference 4:
Meyer, U., et al., Biochemistry 39(12):3461-3471 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-397

List Size:
400 ul

List Price:

$495.00

Datasheet
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