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Antibody used in IHC on Human Lung.
Antibody used in WB on Human HepG2 at 1 ug/ml.
Antibody used in IHC on Human Lung.

GPNMB Antibody

CATALOG NUMBER: 29-998

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GPNMB.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
GPNMB antibody can be used for detection of GPNMB by ELISA at 1:312500. GPNMB antibody can be used for detection of GPNMB by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
60 kDa, 64 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store GPNMB antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
GPNMB
Additional Names:
GPNMB, HGFIN, NMB
Protein Accession Number:
NP_002501
Protein Gi Number:
4505405
Ncbi Gene Id Number:
10457
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GPNMB is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential.The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Oh, J.H., (2005) Mamm. Genome 16 (12), 942-954.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-998

List Size:
100 ul

List Price:

$519.00

Datasheet
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