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Western Blot at 1:500 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:500 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.

GPNMB Antibody

CATALOG NUMBER: 59-048

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GPNMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-569 amino acids from the C-terminal region of human GPNMB.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:500
Predicted Molecular Weight:
64 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GPNMB
Additional Names:
Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, HGFIN, NMB
Protein Accession Number:
Q14956
Protein Gi Number:
61252504
Ncbi Gene Id Number:
10457
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a type I transmembraneglycoprotein which shows homology to the pMEL17 precursor, amelanocyte-specific protein. GPNMB shows expression in the lowlymetastatic human melanoma cell lines and xenografts but does notshow expression in the highly metastatic cell lines. GPNMB may beinvolved in growth delay and reduction of metastatic potential. Twotranscript variants encoding different isoforms have been found forthis gene.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Williams, M.D., et al. Melanoma Res. 20(3):184-190(2010)
Background Reference 3:
Hoashi, T., et al. FASEB J. 24(5):1616-1629(2010)
Background Reference 4:
Rose, A.A., et al. Clin. Cancer Res. 16(7):2147-2156(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-048

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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