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Western blot analysis of GPR17 Antibody in MDA-MB435 cell line lysates (35ug/lane)
GPR17 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of GPR17 Antibody in MDA-MB435 cell line lysates (35ug/lane)

GPR17 Antibody

CATALOG NUMBER: 64-147

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GPR17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-258 amino acids from the Central region of human GPR17.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GPR17
Additional Names:
Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT receptor, G-protein coupled receptor 17, P2Y-like receptor, R12, GPR17
Protein Accession Number:
Q13304
Protein Gi Number:
21264435
Ncbi Gene Id Number:
2840
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Members of the G protein coupled receptor (GPCR) superfamily contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. The organization of the GPR17 gene differs from that of many other GPCRs in that the open reading frame is distributed on 2 exons; an additional exon contains the 5 prime untranslated region. Human GPR17 is expressed as 2.3 and 6.3 kb mRNAs exclusively in brain. The 2 transcripts appear to represent alternatively polyadenylated variants. Based on protein sequence homology and the conservation of certain key residues, GPR17 appears to be closely related to the P2Y family of GPCRs. There are two nemed isoforms.
Background Reference 1:
Pugliese, A.M., et al. Am. J. Physiol., Cell Physiol. 297 (4), C1028-C1040 (2009)
Background Reference 2:
Parravicini, C., et al. BMC Bioinformatics 9, 263 (2008)
Background Reference 3:
Ciana, P., et al. EMBO J. 25(19):4615-4627(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-147

List Size:
400 ul

List Price:

$495.00

Datasheet
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