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Western blot analysis of GPR3 in EL4 cell lysate with GPR3 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of GPR3 in human spleen tissue with GPR3 antibody at 2.5 μg/mL.
Immunofluorescence of GPR3 in Human Spleen cells with GPR3 antibody at 20 μg/mL.
Western blot analysis of GPR3 in EL4 cell lysate with GPR3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

GPR3 Antibody

CATALOG NUMBER: 5175

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GPR3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human GPR3.
The immunogen is located within the last 50 amino acids of GPR3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GPR3 antibody can be used for detection of GPR3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 36 kDa
Observed: 36 kDa
Purification:
GPR3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GPR3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GPR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GPR3
Additional Names:
GPR3 Antibody: ACCA, ACCA, G-protein coupled receptor 3, ACCA orphan receptor
Protein Accession Number:
P46089
Protein Gi Number:
1170006
Ncbi Gene Id Number:
2827
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GPR3 Antibody: GPR3, also known as ACCA, is a G-protein coupled receptor that constitutively activates adenylate cyclase and is highly expressed in the central nervous system. Overexpression of GPR3 stimulates the production of amyloid-beta peptide (Abeta), the deposition of which is one of the pathological hallmarks of Alzheimer 's disease (AD), while the ablation of GPR3 prevented the accumulation of Abeta in vitro and in an AD mouse model. This is of particular interest because of the proximity of a reported candidate Alzheimer 's disease (AD) locus, suggesting that GPR3 may be a potential therapeutic target for the treatment of AD. GPR3 has also been shown to block the proliferation of cerebellar granule cell precursors (GCP) during postnatal development by inhibiting the Shh-induced proliferation of GCP, indicating that GPR3 activation may represent one of the signals that triggers the postnatal cell cycle exit and terminal differentiation of GPCs.
Background Reference 1:
Marchesse A, Docherty JM, Nguyen T, et al. Cloning of human genes encoding novel G protein-coupled receptors. Genomics1994; 23:609-18.
Background Reference 2:
Eggerickx D, Denef JF, Labbe O, et al. Molecular cloning of an orphan G-protein-coupled receptor that constitutively activates adenylate cyclase. Biochem. J.1995; 309:837-43.
Background Reference 3:
Thathiah A, Spittaels K, Hoffmann M, et al. The orphan G protein-coupled receptor 3 modulates amyloid-beta peptide generation in neurons. Science2009; 323:946-951.
Background Reference 4:
Blacker D, Bertram L, Saunder AJ, et al. Results of a high-resolution genome screen of 437 Alzheimer's disease families. Hum. Mol. Genet.2003; 12:23-32.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5175

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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