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45-694 (0.01ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-694 (0.01ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GPR40 Antibody

CATALOG NUMBER: 45-694

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CAARTQGGKSQK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Breast lysates (calculated MW of 31.5kDa according to NP_005294.1). Recommended concentration: 0.01-0.03ug/ml.
Positive Control 1:
Cat. No. 1311 - Human Breast Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FFAR1
Additional Names:
FFAR1, GPR40, G protein-coupled receptor 40 , HGNC:4498, free fatty acid receptor 1, FFA1R, GPCR40
Protein Accession Number:
NP_005294.1
Protein Gi Number:
4885327
Ncbi Gene Id Number:
2864
Background Reference 1:
Hardy S, St-Onge GG, Joly E, Langelier Y, Prentki M. Oleate promotes the proliferation of breast cancer cells via the G protein-coupled receptor GPR40. J Biol Chem. 2005 Apr 8;280(14):13285-91. Epub 2005 Feb 4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-694

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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