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Western Blot at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
GPR45 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPR45 Antibdy for immunohistochemistry.
Western Blot at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.

GPR45 Antibody

CATALOG NUMBER: 56-301

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This GPR45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-149 amino acids from the Central region of human GPR45.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GPR45
Additional Names:
Probable G-protein coupled receptor 45, PSP24-1, PSP24-alpha, GPR45
Protein Accession Number:
Q9Y5Y3
Protein Gi Number:
82654940
Ncbi Gene Id Number:
11250
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This intronless gene encodes a member of the G protein-coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. This protein may function in the central nervous system.
Background Reference 1:
Salonen, J.T., et al. Am. J. Hum. Genet. 81(2):338-345(2007) Wu, C., et al. Proteomics 7(11):1775-1785(2007) Hillier, L.W., et al. Nature 434(7034):724-731(2005) Kawasawa, Y., et al. Biochem. Biophys. Res. Commun. 276(3):957-964(2000) Kawasawa, Y., et al. Biochem. Biophys. Res. Commun. 276(3):952-956(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-301

List Size:
400 ul

List Price:

$495.00

Datasheet
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