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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis in A549 cell line lysates (35ug/lane).

GPR77 Antibody

CATALOG NUMBER: 60-836

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GPR77 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-325 amino acids from the C-terminal region of human GPR77.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
C5AR2
Additional Names:
C5a anaphylatoxin chemotactic receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5AR2, C5L2, GPR77
Protein Accession Number:
Q9P296
Protein Gi Number:
20137533
Ncbi Gene Id Number:
27202
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The anaphylatoxins C3a (see MIM 120700), C4a (see MIM120810), and C5a are cationic fragments generated during thecomplement cascade that participate in host defense. In the case ofinappropriate complement activation, anaphylatoxins may be involvedin autoimmunity and sepsis. C5L2 is coexpressed with the C5areceptor, C5AR (C5R1; MIM 113995), on polymorphonuclear neutrophilsand may modulate C5AR activity (Gerard et al., 2005 [PubMed16204243]).
Background Reference 1:
Bamberg, C.E., et al. J. Biol. Chem. 285(10):7633-7644(2010)
Background Reference 2:
Cui, W., et al. Mol. Immunol. 46(16):3207-3217(2009)
Background Reference 3:
Cui, W., et al. Mol. Immunol. 46(15):3086-3098(2009)
Background Reference 4:
Scola, A.M., et al. Mol. Immunol. 46(6):1149-1162(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-836

List Size:
400 ul

List Price:

$495.00

Datasheet
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