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Western blot analysis of GPRASP1 in A-20 cell lysate with GPRASP1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of GPRASP1 in human breast carcinoma tissue with GPRASP12 antibody at 5 μg/ml.
Immunofluorescence of GPRASP1 in human breast carcinoma tissue with GPRASP1 antibody at 20 μg/ml.
Western blot analysis of GPRASP1 in A-20 cell lysate with GPRASP1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

GPRASP1 Antibody

CATALOG NUMBER: 7987

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
GPRASP1 antibody was raised against a 15 amino acid peptide near the amino terminus of human GPRASP1.
The immunogen is located within amino acids 260 - 310 of GPRASP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GPRASP1 antibody can be used for detection of GPRASP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
GPRASP1 antibody is human and mouse reactive. This antibody is predicted to not cross-react with GPRASP2.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Predicted Molecular Weight:
Predicted: 153 kDa
Observed: 150 kDa
Purification:
GPRASP1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GPRASP1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GPRASP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
GPRASP1
Additional Names:
G-protein coupled receptor-associated sorting protein 1, GASP, GASP-1, GASP1
Protein Accession Number:
NP_001171656
Protein Gi Number:
296080706
Ncbi Gene Id Number:
9737
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The G protein-coupled receptor associated sorting protein 1 (GPRASP1) is a member of the GPRASP family and is thought to modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors (1,2). GPRASP1 interacts with receptors such as the D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CNR1) and targets these proteins for degradation in lysosomes (2). Recently, it has been shown that increased GPRASP1 levels may act as a potential biomarker for breast cancer (3).
Background Reference 1:
Simonin F, Karcher P, Boeuf JJ, et al. Identification of a novel family of G protein-coupled receptor associated proteins. J. Neurochem. 2004; 89:766-75.
Background Reference 2:
Moser E, Kargl J, Whistler JL, et al. G protein-coupled receptor-associated sorting protein 1 regulates the postendocytic sorting of seven-transmembrane-spanning G protein-coupled receptors. Pharmacology 2010; 86:22-9.
Background Reference 3:
Tuszynski GP, Rothman VL, Zheng X, et al. G-protein coupled receptor-associated sorting protein 1 (GASP-1), a potential biomarker in breast cancer. Exp. Mol. Pathol. 2011; 91:608-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7987

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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