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45-699 (1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-699 (20ug/ml) staining of PFA-perfused cryosection of Porcine Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia.
45-699 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-699 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-699 (1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

GPX3 Antibody

CATALOG NUMBER: 45-699

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog,
Immunogen:
The immunogen for this antibody is: C-NQFGKQEPGENSE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 26kDa band observed in Mouse Kidney and Liver lysates (calculated MW of 27.7kDa according to Mouse NP_001077398.1 [isoform1], 25.4kDa according to Mouse NP_032187.2 [isoform 2] and 25.5kDa according to Human NP_002075.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Colon. Recommended concentration: 5ug/ml. Frozen sections of Porcine Kidney shows vesicular staining of the cytoplasm in PCT epithelial cells. Recommended concentration: 20ug/ml.
Specificity:
This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GPX3
Additional Names:
GPX3, glutathione peroxidase 3 (plasma), GPx-P, GSHPx-3, GSHPx-P , extracellular glutathione peroxidase, plasma glutathione peroxidase 3
Protein Accession Number:
NP_002075.2
Protein Gi Number:
6006001
Ncbi Gene Id Number:
2878
Background Reference 1:
Chu FF, Esworthy RS, Doroshow JH, Doan K, Liu XF. Expression of plasma glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents. Blood. 1992 Jun 15;79(12):3233-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-699

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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