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45-700 (0.1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-700 (0.3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
45-700 (0.1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GPX4 Antibody

CATALOG NUMBER: 45-700

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-EEPLVIEKDLPHY
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3ug/ml.
Specificity:
This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GPX4
Additional Names:
GPX4, glutathione peroxidase 4 (phospholipid hydroperoxidase) , HGNC:4556, PHGPx, snGPx, glutathione peroxidase 4, phospholipid hydroperoxidase, sperm nucleus glutathione peroxidase
Protein Accession Number:
NP_002076.2,NP_001034937.1
Protein Gi Number:
75709200
Ncbi Gene Id Number:
2879
Background Reference 1:
Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN. Mammalian selenoprotein thioredoxin/glutathione reductase: Roles in disulfide bond formation and sperm maturation. J Biol Chem. 2005 May 18;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-700

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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