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45-702 (0.3ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
45-702 (5ug/ml) as the reporter antibody.
45-702 (0.3ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

GPX7 Antibody

CATALOG NUMBER: 45-702

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CNQFGQQEPDSNK
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 20kDa band observed in Human Prostate lysates (calculated MW of 21.0kDa according to NP_056511.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 40kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant GPX7 captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml
Positive Control 1:
Cat. No. 1312 - Human Prostate Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GPX7
Additional Names:
GPX7, glutathione peroxidase 7 [Homo sapiens] , HGNC:4559, CL683, FLJ14777, GPX6, NPGPx, non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase
Protein Accession Number:
NP_056511.2
Protein Gi Number:
15618997
Ncbi Gene Id Number:
2882
Background Reference 1:
Utomo A, Jiang X, Furuta S, Yun J, Levin DS, Wang YC, Desai KV, Green JE, Chen PL, Lee WH. Identification of a novel putative non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) essential for alleviating oxidative stress generated from polyunsaturated fatty acids in breast cancer cells. J Biol Chem. 2004 Oct 15;279(42):43522-9. Epub 2004 Aug 4.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-702

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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