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45-704 (0.3ug/ml) staining of human thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-704 (0.3ug/ml) staining of human thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GRAP Antibody

CATALOG NUMBER: 45-704

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-GFFPRSYVQPVHL
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 26kDa band observed in human thymus lysates (calculated MW of 25.3kDa according to NP_006604.1). Recommended concentration: 0.3-1ug/ml.
Positive Control 1:
Cat. No. 1314 - Human Thymus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GRAP
Additional Names:
GRAP, GRB2-related adaptor protein, growth factor receptor-bound protein 2-related adaptor protein, MGC64880
Protein Accession Number:
NP_006604.1
Protein Gi Number:
5729856
Ncbi Gene Id Number:
10750
Background Reference 1:
Feng GS, Ouyang YB, Hu DP, Shi ZQ, Gentz R, Ni J. Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway. J Biol Chem 1996 May 24;271(21):12129-32

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-704

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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