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45-705 (0.3ug/ml) staining of Jurkat lysate (30ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing GRAP2 and probed with 45-705 (mock transfection in first lane).
45-705 (0.3ug/ml) staining of Jurkat lysate (30ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GRAP2 Antibody

CATALOG NUMBER: 45-705

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-GLFPANYVAPMTR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed lysates of cell line Jurkat (calculated MW of 37.9kDa according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GRAP2
Additional Names:
GRAP2, GRID, P38, GADS, GRPL, GrbX, Mona, GRB2L, GRBLG, GRAP-2, GRB2-related adaptor protein 2, GRB-2-like protein, SH3-SH2-SH3 adaptor molecule, growth factor receptor-binding protein, GRB2-related protein with insert domain, hematopoietic cell-associated adaptor protein GRPL, growth factor receptor-bound protein 2-related adaptor protein 2, RP3-370M22.1, Grf40, Mona
Protein Accession Number:
NP_004801.1
Protein Gi Number:
4758476
Ncbi Gene Id Number:
9402
Background Reference 1:
Qiu M, Hua S, Agrawal M, Li G, Cai J, Chan E, Zhou H, Luo Y, Liu M. Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1. Biochem Biophys Res Commun. 1998 Dec 18;253(2):443-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-705

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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