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Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of GRB10 in rat brain cells with GRB10 antibody at 20 μg/mL.
Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

GRB10 Antibody

CATALOG NUMBER: 6733

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%)
Immunogen:
GRB10 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human GRB10.
The immunogen is located within amino acids 440 - 490 of GRB10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
GRB10 antibody can be used for detection of GRB10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of GRB10 are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Purification:
GRB10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GRB10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GRB10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GRB10
Additional Names:
GRB10 Antibody: RSS, IRBP, MEG1, GRB-IR, Grb-10, GRBIR, KIAA0207, Growth factor receptor-bound protein 10, GRB10 adapter protein
Protein Accession Number:
NP_005302
Protein Gi Number:
48762679
Ncbi Gene Id Number:
2887
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GRB10 Antibody: GRB10 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules and function in diverse cellular processes. GRB10 is a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of GRB10 inhibits tyrosine kinase activity and results in growth suppression. GRB10 can also interact with the L isoform of the proapoptotic protein Bim. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts.
Background Reference 1:
Morrione A. Grb10 proteins in insulin-like growth factor and insulin receptor signaling (review). Int. J. Mol. Med. 2000; 5:151-4.
Background Reference 2:
Liu F and Roth RA. Grb-IR: a SH2-domain-containing protein that binds to the insulin receptor and inhibits its function. Proc. Natl. Acad. Sci. USA 1995; 92:10287-91.
Background Reference 3:
Wick KR, Werner ED, Langlais P, et al. Grb10 inhibits insulin-stimulated insulin receptor substrate (IRS)-phosphatidylinositol 3-kinase/Akt signaling pathway by disrupting the association of IRS-1/IRS-2 with the insulin receptor. J. Biol. Chem. 2003; 278:8460-7.
Background Reference 4:
Hu ZQ, Zhang JY, Ji CN, et al. Grb10 interacts with Bim L and inhibits apoptosis. Mol. Biol. Rep. 2010; 37:3547-52.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6733

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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