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Western blot analysis of lysate from Ramos cell line, using GRB2 Antibody (pY209) at 1:1000.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 Antibody (Y209), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from Ramos cell line, using GRB2 Antibody (pY209) at 1:1000.

GRB2 Antibody

CATALOG NUMBER: 62-216

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Chicken, Mouse, Rat
Immunogen:
This GRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from human GRB2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
25 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GRB2
Additional Names:
Growth factor receptor-bound protein 2, Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2, GRB2, ASH
Protein Accession Number:
P62993
Protein Gi Number:
51702266
Ncbi Gene Id Number:
2885
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GRB2 binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway.
Background Reference 1:
Kondo,A., J. Biol. Chem. 283 (3), 1428-1436 (2008) Morimatsu,M., Proc. Natl. Acad. Sci. U.S.A. 104 (46), 18013-18018 (2007) Martinez,N., Cell. Signal. 19 (11), 2277-2285 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-216

List Size:
400 ul

List Price:

$495.00

Datasheet
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