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46-724 (2ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-724 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-724 (2ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

GRIA4 Antibody

CATALOG NUMBER: 46-724

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-KKLDQREYPGSETP
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx.100kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2kDa according to NP_001070711.2). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 5-10ug/ml.
Specificity:
This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GRIA4
Additional Names:
GRIA4 , glutamate receptor, ionotrophic, AMPA 4 , GLUR4, GLUR4C, GLURD, AMPA-selective glutamate receptor 4
Protein Accession Number:
NP_000820.3,NP_001070711.2,NP_001070712.1
Protein Gi Number:
164419734
Ncbi Gene Id Number:
2893
Background Reference 1:
Earnshaw BA, Bressloff PC. Biophysical model of AMPA receptor trafficking and its regulation during long-term potentiation/long-term depression. J Neurosci. 2006 Nov 22;26(47):12362-73.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-724

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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