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Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
GRIA4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.

GRIA4 Antibody

CATALOG NUMBER: 56-748

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Pr
Immunogen:
This GRIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-325 amino acids from the Central region of human GRIA4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
101 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GRIA4
Additional Names:
Glutamate receptor 4, GluR-4, GluR4, AMPA-selective glutamate receptor 4, GluR-D, Glutamate receptor ionotropic, AMPA 4, GluA4, GRIA4, GLUR4
Protein Accession Number:
P48058
Protein Gi Number:
218512059
Ncbi Gene Id Number:
2893
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glutamate receptors are the predominant excitatoryneurotransmitter receptors in the mammalian brain and are activatedin a variety of normal neurophysiologic processes. These receptorsare heteromeric protein complexes composed of multiple subunits,arranged to form ligand-gated ion channels. The classification ofglutamate receptors is based on their activation by differentpharmacologic agonists. The subunit encoded by this gene belongs toa family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate)-sensitive glutamate receptors, and is subject to RNAediting (AGA->GGA; R->G). Alternative splicing of this gene resultsin transcript variants encoding different isoforms, which may varyin their signal transduction properties. Some haplotypes of thisgene show a positive association with schizophrenia. [provided byRefSeq].
Background Reference 1:
Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009)
Background Reference 2:
Volpi, S., et al.Clin Psychiatry 70(6):801-809(2009)
Background Reference 3:
Kessels, H.W., et al. Neuron 61(3):340-350(2009)
Background Reference 4:
Arai, S., et al. Psychiatr. Genet. 19(1):6-13(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-748

List Size:
400 ul

List Price:

$495.00

Datasheet
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