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42-093 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-093 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

GRIK5 Antibody

CATALOG NUMBER: 42-093

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-GPAGPRELAEHE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx. 26kDa band in Human Brain (Frontal Cortex and Hippocampus) lysates after 1ug/ml antibody staining. Please note that currently we can not find an explanation in the literature for the band we observe given the calculated size of 109kDa according to NP_002079.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GRIK5
Additional Names:
GRIK5, glutamate receptor, ionotropic, kainate 5, EAA2, GRIK2, KA2, excitatory amino acid receptor 2, glutamate receptor KA2
Protein Accession Number:
NP_002079.3
Protein Gi Number:
29029597
Ncbi Gene Id Number:
2901
Background Reference 1:
Fotheringham J, Williams EL, Akhyani N, Jacobson S. Human herpesvirus 6 (HHV-6) induces dysregulation of glutamate uptake and transporter expression in astrocytes. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2008 Jun 3 (2): 105-16.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-093

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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