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45-711 (2ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-711 (2ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

GRK2 Antibody

CATALOG NUMBER: 45-711

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-RDAYREAQQLVQR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80kDa band observed in Daudi cell lysates (calculated MW of 79.7kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48kDa and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-2ug/ml. Primary incubation 1 hour at room temperature.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ADRBK1
Additional Names:
BARK1, GRK2, ADRBK1, adrenergic, beta, receptor kinase 1, BETA-ARK1, beta adrenergic receptor kinase 1
Protein Accession Number:
NP_001610.2
Protein Gi Number:
4501971
Ncbi Gene Id Number:
156
Background Reference 1:
Iaccarino G, Barbato E, Cipolletta E, De Amicis V, Margulies KB, Leosco D, Trimarco B, Koch WJ. Elevated myocardial and lymphocyte GRK2 expression and activity in human heart failure. Eur Heart J. 2005 Sep;26(17):1752-8. Epub 2005 Jul 29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-711

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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