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Western blot analysis of GRK6 in rat small intestine tissue lysate with GRK6 antibody at 1 μg/ml.
Immunohistochemistry of GRK6 in human small intestine tissue with GRK6 antibody at 5 μg/mL.
Immunofluorescence of GRK6 in human small intestine tissue with GRK6 antibody at 20 μg/mL.
Western blot analysis of GRK6 in rat small intestine tissue lysate with GRK6 antibody at 1 μg/ml.
Trial Size

GRK6 Antibody

CATALOG NUMBER: 7641

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GRK6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human GRK6.
The immunogen is located within the last 50 amino acids of GRK6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GRK6 antibody can be used for detection of GRK6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
GRK6 antibody is human, mouse and rat reactive. Multiple isoforms of GRK6 are known to exist. This antibody is predicted to not cross-react with other members of the GRK protein family.
Positive Control 1:
Cat. No. 1468 - Rat Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 63 kDa
Observed: 63 kDa
Purification:
GRK6 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GRK6 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GRK6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
GRK6
Additional Names:
GRK6 Antibody: GPRK6, GPRK6, G protein-coupled receptor kinase 6, G protein-coupled receptor kinase GRK6
Protein Accession Number:
NP_001004106
Protein Gi Number:
51896039
Ncbi Gene Id Number:
2870
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The G protein–coupled receptor kinases (GRKs) are a versatile family of kinases that play a critical role in cancer metastasis through their regulation of G-protein coupled receptors (GPCRs) involved in growth factor mediated cell migration (1,2). Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state (1). GRK6 is one of 7 members of the GRK serine/threonine kinase subfamily, which has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 (3,4), and the insulin-like growth factor signaling pathway (4). GRK6 may also play a role in immune system function (5).
Background Reference 1:
Inglese J, Freedman NJ, Koch WJ, et al. Structure and mechanism of the G protein-coupled receptor kinases. J. Biol. Chem. 1993; 268:23735-8.
Background Reference 2:
Raghuwanshi SK, Smith N, Rivers EJ, et al. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J. Immunol. 2013; 190:5329-36.
Background Reference 3:
Benovic JL and Gomez J. Molecular cloning and expression of GRK6. A new member of the G protein-coupled receptor kinase family. J. Biol. Chem. 1993; 268:19521-7.
Background Reference 4:
Chen M, Philipp M, Wang J, et al. G Protein-coupled receptor kinases phosphorylate LRP6 in the Wnt pathway. J. Biol. Chem. 2009; 284:35040-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7641

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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