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IHC testing of FFPE human pituitary gland with Growth Hormone antibody (clone GH/1371). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free Growth Hormone Antibody (clone GH/1371). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human pituitary gland with Growth Hormone antibody (clone GH/1371). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Growth Hormone Antibody [GH/1371]

CATALOG NUMBER: 33-738

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Amino acids 58-187 from the human protein were used as the immunogen for this Growth Hormone antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
Titration of the Growth Hormone antibody may be needed for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
Growth Hormone
Additional Names:
GH1, Growth hormone, GH, GHN, IGHD1B, GH-N, Pituitary growth hormone, Growth hormone 1, HGH-N, Somatotropin
Ncbi Gene Id Number:
2688
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Growth hormone (GH), also known as somatotropin (or as human growth hormone [hGH or HGH] in its human form), is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. It is thus important in human development. It is a type of mitogen which is specific only to certain kinds of cells. Growth hormone is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic cells within the lateral wings of the anterior pituitary gland.

GH is a stress hormone that raises the concentration of glucose and free fatty acids. It also stimulates production of IGF-1.

The major isoform of the human growth hormone is a protein of 191 amino acids and a molecular weight of 22,124 daltons. The structure includes four helices necessary for functional interaction with the GH receptor. It appears that, in structure, GH is evolutionarily homologous to prolactin and chorionic somatomammotropin. [Wiki]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-738

List Size:
100 ug

List Price:

$626.00

Datasheet
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