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Western blot analysis of extracts of various cell lines, using HSPA5 antibody (13-101) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using GRP78 / BiP antibody (13-101) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using HSPA5 antibody (13-101) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

GRP78 / BiP Antibody

CATALOG NUMBER: 13-101

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human GRP78 / BiP (NP_005338.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
U-87MG
Positive Control 3:
HepG2
Positive Control 4:
HeLa
Predicted Molecular Weight:
Observed: 78kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HSPA5
Additional Names:
HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP-78, GRP78
Ncbi Gene Id Number:
3309
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-101

List Size:
100 uL

List Price:

$436.00

Datasheet
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