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Antibody used in WB on Human Heart at 0.2-1 ug/ml.
Antibody used in WB on Human Heart at 0.2-1 ug/ml.

GRPEL2 Antibody

CATALOG NUMBER: 26-723

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GRPEL2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
GRPEL2 antibody can be used for detection of GRPEL2 by ELISA at 1:312500. GRPEL2 antibody can be used for detection of GRPEL2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10407 - Fetal Heart Tissue Lysate
Predicted Molecular Weight:
25 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store GRPEL2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
GRPEL2
Additional Names:
GRPEL2, DKFZp451C205, FLJ23713, FLJ33918, Mt-GrpE#2
Protein Accession Number:
NP_689620
Protein Gi Number:
40255109
Ncbi Gene Id Number:
134266
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GRPEL2 is an essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. GRPEL2 seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. GRPEL2 stimulates ATPase activity of mt-HSP70. GRPEL2 may also serve to modulate the interconversion of oligomeric (inactive) and monomeric (active) forms of mt-HSP70.
Background Reference 1:
Naylor, D.J., (1998) J. Biol. Chem. 273 (33), 21169-21177.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-723

List Size:
100 ul

List Price:

$519.00

Datasheet
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