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Western blot analysis of GRTP1 in K562 cell lysate with GRTP1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of GRTP1 in K562 cells with GRTP1 antibody at 2.5 μg/mL.
Immunofluorescence of GRTP1 in K562 cells with GRTP1 antibody at 20 μg/mL.
Western blot analysis of GRTP1 in K562 cell lysate with GRTP1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

GRTP1 Antibody

CATALOG NUMBER: 4589

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GRTP1 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human GRTP1.
The immunogen is located within the first 50 amino acids of GRTP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
GRTP1 antibody can be used for detection of GRTP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
GRTP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GRTP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GRTP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GRTP1
Additional Names:
GRTP1 Antibody: TBC1D6, TBC1D6, Growth hormone-regulated TBC protein 1, TBC1 domain family member 6
Protein Accession Number:
EAX09206
Protein Gi Number:
119629611
Ncbi Gene Id Number:
79774
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GRTP1 Antibody: Growth hormone regulated TBC protein 1 (GRTP1) was identified through microarray analysis as a gene whose expression is regulated by growth hormone (GH). It contains the TBC signature motif of GTPase activator proteins of Rab-like small GTPases. GRTP1 mRNA is expressed at highest levels in testes, and is also present in kidney, lung, liver, and intestine. Administration of GH to mice resulted in an increase in GRTP1 mRNA in testes, but a decrease of GRTP1 mRNA in kidney and liver. GRTP1 was identified as an HIV dependency factor (HDF), suggesting that GRTP1 may be an important drug target in HIV treatment. At least three isoforms of GRTP1 are known to exist; this antibody should recognize all isoforms.
Background Reference 1:
Lu C, Kasik J, Stephan DA, et al. Grtp1, a novel gene regulated by growth hormone. Endocriniology2001; 142:4568-71.
Background Reference 2:
Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4589

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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