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Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Daudi whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Daudi whole cell lysate Lysates/proteins at 20 ug per lane.

GSG2 Antibody

CATALOG NUMBER: 63-374

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Haspin GSG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323~352 amino acids from the central region of human haspin.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
88 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GSG2
Additional Names:
Serine/threonine-protein kinase haspin, Germ cell-specific gene 2 protein, H-haspin, Haploid germ cell-specific nuclear protein kinase, GSG2
Protein Accession Number:
Q8TF76
Protein Gi Number:
296439330
Ncbi Gene Id Number:
83903
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Post-translational modifications of conserved N-terminal tail residues in histones regulate many aspects of chromosome activity. Mitotic phosphorylation of H3 Thr 3 occurs in prophase and dephosphorylation during anaphase. Haspin, a dual serine/threonine kinase, plays an important role in regulation of chromosome and spindle function during mitosis and meiosis via its function in phosphorylation of the threonine residue in the third position of histone 3 (Thr3).
Background Reference 1:
Dai, Jun et al. Genes Dev 19:472-88 (2005).
Background Reference 2:
Higgins, J.M., Gene 267(1):55-69 (2001).
Background Reference 3:
Tanaka, H., et al., Mol. Hum. Reprod. 7(3):211-218 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-374

List Size:
400 ul

List Price:

$495.00

Datasheet
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