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Western blot analysis using anti - human GSK3 alpha nonoclonal antibody against truncated GSK3 alpha recombinant protein and A549 lysate.
Immunohistochemical analysis of paraffin - embedded human lung carcinoma showing cytoplasmic localization using GSK3 alpha antibody with DAB staining.
Immunohistochemical analysis of paraffin - embedded human skin carcinoma showing cytoplasmic localization using GSK3 alpha antibody with DAB staining.
Western blot analysis using anti - human GSK3 alpha nonoclonal antibody against truncated GSK3 alpha recombinant protein and A549 lysate.

GSK3alpha Antibody [6G2F1 / 6G2F2]

CATALOG NUMBER: 32-164

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant GSK3 alpha expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
GSK3 alpha monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GSK3A
Additional Names:
GSK-3 alpha,
Protein Accession Number:
P49840
Protein Gi Number:
12644292
Ncbi Gene Id Number:
2931
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GSK3 alpha (Glycogen synthase kinase 3 alpha), with 483-amino acid protein (about 53 kDa), belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily,GSK3 subsubfamily, which also includes GSK3 beta.GSK3 is a multifunctional serine/threonine kinase that is usually inactivated by serine phosphorylation in response to extracellular cues. GSK3 is a key regulator of numerous signalling pathways, including cellular responses to Wnt, receptor tyrosine kinases and G-protein-coupled receptors and is involved in a wide range of cellular processes, ranging from glycogen metabolism to cell cycle regulation and proliferation.GSK3 alpha is implicated in the control of several regulatory proteins including glycogen synthase and transcription factors.
Background Reference 1:
Bradley W. Doble and James R. Woodgett. J. Cell Sci., Apr 2003; 116: 1175.
Background Reference 2:
C. Laura Sayas , Aafke Ariaens, Bas Ponsioen. Mol. Biol. Cell, Apr 2006; 17: 1834 – 1844.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-164

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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