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Western blot analysis using GSK3 monoclonal antibody against truncated GSK3alpha recombinant protein
Immunofluorescense analysis hof Hela cells using GSK3a antibody showing cytoplasmic localizaiton.
Western blot analysis using GSK3 monoclonal antibody against truncated GSK3alpha recombinant protein

GSK3alpha Antibody [9D5G1]

CATALOG NUMBER: 32-163

Clonality:
Monoclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant GSK3 alpha expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Western Blot:1:500 - 1:2,000
IF(1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
GSK3 alpha monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GSK3A
Additional Names:
GSK-3 alpha,
Protein Accession Number:
P49840
Protein Gi Number:
12644292
Ncbi Gene Id Number:
2931
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily; GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.
Background Reference 1:
Mendez P, Garcia-Segura LM. Endocrinology. 2006 Jun;147(6):3027-39. Epub 2006 Feb 23.
Background Reference 2:
Bianchi M, De Lucchini S, et, al. Biochem J. 2005 Oct 15;391(Pt 2):359-70.
Background Reference 3:
De Servi B, Hermani A, Medunjanin S, Mayer D. Oncogene. 2005 Jul 21;24(31):4946-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-163

List Size:
0.1 mg

List Price:

$432.00

Datasheet
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