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Western blot analysis of extracts of various cell lines, using GSK3B antibody (22-038) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts from normal (control) and GSK3B knockout (KO) HeLa cells, using GSK3B antibody (22-038) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using GSK3B antibody (22-038) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 22Rv1 cells, using 3 ug GSK3B antibody (22-038). Western blot was performed from the immunoprecipitate using GSK3B antibody (22-038) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using GSK3B antibody (22-038) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
KO

GSK3B Antibody, KO Validated

CATALOG NUMBER: 22-038

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GSK3B (NP_001139628.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:1000
IF: 1:50 - 1:100
IP: 1:50 - 1:100
Positive Control 1:
LO2
Positive Control 2:
HeLa
Positive Control 3:
22Rv1
Positive Control 4:
U-251MG
Predicted Molecular Weight:
Observed: 46kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GSK3B
Additional Names:
GSK3B, GSK-3 beta, GSK3 beta, GSK3beta, GSK3beta isoform, TPKI, GSK 3beta, Tau protein kinase I, ZGSK-3beta
Ncbi Gene Id Number:
2932
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-038

List Size:
100 uL

List Price:

$436.00

Datasheet
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