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Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using GSK3β (Phospho-Tyr216).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Tyr216).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using GSK3β (Phospho-Tyr216).
Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using GSK3β (Phospho-Tyr216).

GSK3beta (phospho Tyr216) Antibody

CATALOG NUMBER: 79-231

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GSK3beta (Phospho-Tyr216) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 216 (V-S-Y (p) -I-C) derived from Human GSK3β.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of GSK3β only when phosphorylated at tyrosine 216.
Predicted Molecular Weight:
46 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
GSK3B
Additional Names:
FLK1, VEGFR2, Factor A, GSK-3 alpha/beta, kinase GSK3-alpha/beta
Protein Accession Number:
NP_001139628.1
Protein Gi Number:
225903437
Ncbi Gene Id Number:
2932
Background:
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
Background Reference 1:
Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13; 137 (1-2): 193-201Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02; 316 (2): 411-415Fan G, et al. J Biol Chem 2003 Dec 26; 278 (52): 52432-52436Liao X, et al. Mol Cancer Ther 2003 Nov; 2 (11): 1215-1222

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-231

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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