Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis using GST monoclonal antibody against GST recombinant protein.
Western blot analysis using GST monoclonal antibody against GST recombinant protein.

GST Antibody [6G9B9]

CATALOG NUMBER: 32-166

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
GST
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
GST
Immunogen:
Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:Dilution 1:2000 - 5,000
ELISA:Proposed dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
GST monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
gst
Additional Names:
gst, b1635, JW1627, GST B1-1
Protein Accession Number:
P0A9D2
Protein Gi Number:
71159393
Ncbi Gene Id Number:
12931264
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
Background Reference 1:
Singlestep purification of polypeptides expressed in Escherichia colias fusions with glutathione Stransferase. 1988. Gene 67: 31-40.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-166

List Size:
0.1 mg

List Price:

$249.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369