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Western blot analysis using GSTP1 monoclonal antibody against full - length GSTP1 recombinant protein.
Immunohistochemical analysis of paraffin - embedded human prostate tissue showing cytoplasmic location using GSTP1 antibody with DAB staining.
Western blot analysis using GSTP1 monoclonal antibody against full - length GSTP1 recombinant protein.

GSTP1 Antibody [3F2C2]

CATALOG NUMBER: 32-167

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant GSTP1 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
GSTP1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GSTP1
Additional Names:
FAEES3, GST3, GST class-piPI, DFN7, GSTP, FAEES3, HEL-S-22
Protein Accession Number:
P09211
Protein Gi Number:
121746
Ncbi Gene Id Number:
2950
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GSTP1 (glutathione-S-transferase, pi 1), also called GST3/DFN7, which is located on chromosome 11q13 , is a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 act like a tumor suppressor gene, which when inactivated leads to tumor growth, and the -class glutathione S-transferase is commonly inactivated by somatic CpGisland hypermethylation in cancers of the prostate, liver, and breast. Methylation of regulatory sequences at the GSTP1 gene locus is found in the vast majority (>90%) of prostate carcinomas and is associated with transcriptional down-regulation.
Background Reference 1:
Kimihiko Satoh, Ken Itoh, Masayuki Yamamoto. 2002. Carcinogenesis. 23: 457 - 462.
Background Reference 2:
Kimihiko Satoh, Ken Itoh, Masayuki Yamamoto. 2002. Carcinogenesis. 23: 457 - 462.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-167

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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