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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Western blot analysis in mouse kidney tissue lysates (35ug/lane).

GTF2A1 Antibody

CATALOG NUMBER: 59-357

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This GTF2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the C-terminal region of human GTF2A1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GTF2A1
Additional Names:
Transcription initiation factor IIA subunit 1, General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42, Transcription initiation factor IIA alpha chain, TFIIA p35 subunit, Transcription initiation factor IIA beta chain, TFIIA p19 subunit, GTF2A1, TF2A1
Protein Accession Number:
P52655
Protein Gi Number:
1711663
Ncbi Gene Id Number:
2957
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Accurate transcription initiation on TATA-containing classII genes involves the ordered assembly of RNA polymerase II(POLR2A; MIM 180660) and several general initiation factors(summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One ofthese factors is TFIIA, which when purified from HeLa extractsconsists of 35-, 19-, and 12-kD subunits.
Background Reference 1:
Huang, Y.W., et al. Oncol. Rep. 22(4):853-861(2009)
Background Reference 2:
Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
Background Reference 3:
Hieb, A.R., et al. J. Mol. Biol. 372(3):619-632(2007)
Background Reference 4:
Lee, K.A., et al. Cytokine 38(2):96-100(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-357

List Size:
400 ul

List Price:

$495.00

Datasheet
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