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Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.

GTF2A1L Antibody

CATALOG NUMBER: 57-690

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This GTF2A1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-428 amino acids from the C-terminal region of human GTF2A1L.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GTF2A1L
Additional Names:
TFIIA-alpha and beta-like factor, General transcription factor II A, 1-like factor, GTF2A1L, ALF, GTF2A1LF
Protein Accession Number:
Q9UNN4
Protein Gi Number:
327478545
Ncbi Gene Id Number:
11036
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The assembly and stability of the RNA polymerase IItranscription pre-initiation complex on a eukaryotic core promoterinvolve the effects of TFIIA on the interaction betweenTATA-binding protein (TBP) and DNA. This gene encodes a germcell-specific counterpart of the large (alpha/beta) subunit ofgeneral transcription factor TFIIA that is able to stabilize thebinding of TBP to DNA and may be uniquely important to testisbiology. Alternative splicing for this locus has been observed andtwo variants, encoding distinct isoforms, have been identified.Co-transcription of this gene and the neighboring upstream genegenerates a rare transcript (SALF), which encodes a fusion proteincomprised of sequence sharing identity with each individual geneproduct.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Kim, M., et al. J. Biol. Chem. 281(45):34288-34298(2006)
Background Reference 3:
Howe, M.L., et al. Stem Cells Dev. 15(2):175-190(2006)
Background Reference 4:
Huang, M., et al. Int. J. Mol. Med. 17(4):599-604(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-690

List Size:
400 ul

List Price:

$495.00

Datasheet
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